scFv Antibody Engineering

A single chain variable fragment (scFv) is a fusion of the variable regions of the heavy (VH) and light chains (VL) of antibodies, connected with a short linker peptide of 10 to 25 amino acids. Made as a single protein, scFv-Fc retains the specificity of the original immunoglobulin, and in many applications outperforms whole IgG in construction speed, production yield, and engineering flexibility. LakePharma utilizes scFv in its antibody cloning and phage display optimization platforms.

LakePharma has developed a highly efficient process to engineer scFv-Fc fusions and produce large quantities of purified protein from CHO cells. The scFv-Fc proteins usually retain the same binding affinity and specificity as full-length antibodies, and therefore this format is often used to confirm antigen binding after sequencing a variable region from a hybridoma. The scFv format is also used in molecular evolution processes for antibody affinity maturation using phage display. With its optimized scFv expression vectors and its proprietary scFv construction technologies, LakePharma leads the industry in constructing and producing scFv antibodies.