Catalog #63120 - N-glycan Analysis by LC-MS (Trypsin Digestion)

Title:
N-glycan Analysis by LC-MS (Trypsin Digestion)

Description:

N-glycan Analysis by LC-MS (Trypsin Digestion)

  • PTM analysis will identify relative quantities of glycans, not absolute quantities
  • LP will perform PTM analysis with Trypsin Digestion.
  • The standard protocol for PTM analysis at LP includes the below 9 glycans for analysis. If client wants other glycans tested, an additional charge may apply and client must let LP know this before project initiation. 
    • G0, G0F, G1, G1F, G2, G2F, Man5, G2FSA, G2FSA2

Scope:

1. The antibody/protein of interest is denatured, reduced, and digested with one or more proteolytic enzymes, such as trypsin.

2. The resulting peptide mixtures are submitted to a reverse-phase HPLC coupled to a mass spectrometer.

3. The mass spectra is compared against the target protein sequence and the glycans of interests are identified (if present).


Note: the above quoted prices are based on analysis using LakePharma’s standardized methods, proven to work for a majority of antibodies and proteins. Method development may be necessary for atypical proteins with unique properties for an additional fee.


Deliverable:
Study report.

Material Required:
  • ≥ 100µg of purified protein at ≥ 1mg/mL
  • Target protein sequence
  • Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to LakePharma for analysis

Timeline:
2 week

Price:
$1,320