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Catalog #63120 - N-glycan Analysis by LC-MS (Trypsin Digestion)
Title:
N-glycan Analysis by LC-MS (Trypsin Digestion)
Description:
N-glycan Analysis by LC-MS (Trypsin Digestion)
- PTM analysis will identify relative quantities of glycans, not absolute quantities
- LP will perform PTM analysis with Trypsin Digestion.
- The standard protocol for PTM analysis at LP includes the below 9 glycans for analysis. If client wants other glycans tested, an additional charge may apply and client must let LP know this before project initiation.
- G0, G0F, G1, G1F, G2, G2F, Man5, G2FSA, G2FSA2
Scope:
1. The antibody/protein of interest is denatured, reduced, and digested with one or more proteolytic enzymes, such as trypsin.
2. The resulting peptide mixtures are submitted to a reverse-phase HPLC coupled to a mass spectrometer.
3. The mass spectra is compared against the target protein sequence and the glycans of interests are identified (if present).
Note: the above quoted prices are based on analysis using LakePharma’s standardized methods, proven to work for a majority of antibodies and proteins. Method development may be necessary for atypical proteins with unique properties for an additional fee.