Catalog #64005 - N-terminal Sequencing (5 residue)

Title:
N-terminal Sequencing (5 residue)

Description:
N-terminal Sequencing (5 residue)

Scope:
  1. The band(s) of interest is excised and submitted to an ABI 494 Protein Sequencer.
  2. In the event, the N-terminus of the protein is blocked and no sequences are obtained, the full project price is still invoiced.

Note: the above-quoted prices are based on analysis using LakePharma’s standardized methods, proven to work for a majority of antibodies and proteins.


Deliverable:
Edman degradation cycle chromatogram with calls for each cycle

Material Required:
  • PVDF membrane (Coomassie Blue or Ponceau S stained) cutout of target bands 
    • Please load at least 5 µg for SDS-PAGE transfer onto PVDF blot for submission
    • If liquid sample will be provided, the service SKU-64000 will be performed and will require 100 µL at > 0.5 mg/mL
  • Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to LakePharma for analysis.

Timeline:
2 Weeks

Price:
$500