Antibody Humanization

Many monoclonal antibodies are discovered from non-human species.  Of the 107 antibody-based therapeutics approved for use in the USA and/or Europe, approximately 15% are murine and 12% are chimeric.  Because non-humanized antibodies and chimeras (human Fc backbone) are often highly immunogenic, the advent of human-antibody producing transgenic animals and humanization techniques can lower the risk of patient immunogenicity.  Over 70% of the approved antibody therapeutics are fully human or humanized.

LakePharma has developed an efficient platform for complementarity-determining regions (CDR) grafting, which involves transferring the antibody (CDRs) into a proper human antibody framework while retaining its original affinity and specificity.  Using LakePharma's T20 Score Analyzer tool, users are able to calculate a monoclonal antibody humanness score to distinguish between human and non-human antibodies. If you are seeking both humanization and affinity maturation, the LakePharma HuMAT™ platform leverages our capabilities in CDR grafting and display discovery to offer a one-step antibody humanization and affinity maturation with time and cost savings, all while delivering consistent high-quality antibody leads.

Humanization workflow at LakePharma

Sequence AnalysisGenerating human antibody variantsHumanized variant analysis in antibody humanization

Humanization and HuMAT™ projects are customizable and the number of molecules can be increased. 

Client Testimonial: Tri-Institutional Therapeutics Discovery Institute and LakePharma: A Partnership in Antibody Discovery to Enable Innovative Therapeutics

Download our Antibody Discovery and Engineering Services Brochure by clicking here

Discovery of Potent, Functional Anti-BTLA, and Anti-TIGIT Monoclonal Antibodies using Hybridoma and Phage Display Platform webinar recording available NOW!

Catalog # Name Timeline Price
1019 HuMAT™ Antibody Humanization and Affinity Maturation 4 to 6 months Request
24401 Antibody Humanization and Affinity Measurement 8 to 9 weeks Request