Catalog #24401 - Antibody Humanization and Affinity Measurement

Title:
Antibody Humanization and Affinity Measurement

Description:
Humanization and Affinity Measurement

Scope:

1. Antibody sequence analysis and homology modeling of mAb 3D structure.

2. Identification of key positions supporting CDR loop structure and VH-VL interface.

3. Design humanized variants (3VH, 3VL).

4. Assess the humanness of humanized variants by T20 humanness score developed at LakePharma. [Sean H Gao, Kexin Huang, Hua Tu, and Adam S Adler. (2013) Monoclonal antibody humanness score and its applications. BMC Biotechnology, 13:55]

5. Construct and express nine humanized antibodies (each combination of three designed heavy chains and three designed light chains) as well as a chimeric version (rodent variable regions, human constant regions) at the 10 mL scale in CHO cells (TunaCHO™ Process).

6. Assess expression levels and measure affinity by Octet, or competitive binding and compare to chimera.


Deliverable:
1. Study report, including sequences of the humanized antibodies. 2. All remaining purified antibodies.

Material Required:
1. Sequences of VL and VH. 2. Antigen and assay for measuring binding and affinity.

Timeline:
8 to 9 weeks

Price:
$52,000