Antibody Reformatting & Production

Antibody V region genes (VH and VL) from a given antibody can be reformatted to encode a different constant region, including Fab. Multiple species and isotypes are available, with the option of synthesizing the constant region of choice. These expression constructs are transiently transfected into HEK293 cells at various scales followed by protein purification. The services listed below use our premium transfection system, generating higher yields for a smaller volume production. Transient transfection into CHO cells is available on request.

Please contact LakePharma at inquiries@lakepharma.com for custom quotes and details.

New - Intact mass spec analysis for all recombinant antibody productions. 

Cat.# Service Name Timeline Size Price Quantity Request
27600 Molecular construction of IgG expression vectors, 0.03 liter transient production in HEK293 cells (Tuna293™ Process) and protein A purification (template DNA provided by client) 4-5 weeks 0.03 liter $2400
27610 Molecular construction of IgG expression vectors, 0.03 liter transient production in HEK293 cells (Tuna293™ Process) and protein A purification (synthesize variable regions) 4-5 weeks 0.03 liter $2900
27620 Molecular construction of IgG expression vectors, 0.03 liter transient production in HEK293 cells (Tuna293™ Process) and protein A purification (synthesize whole IgG genes) 4-5 weeks 0.03 liter $3400
27625 0.03 liter transient production in HEK293 cells (Tuna293™ Process) and protein A purification 2-3 weeks 0.03 liter $1000

1. Antibody genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

2. Pilot small-scale (0.03 liter) transient transfection into 293 or CHO is performed, followed by protein A purification to assess expression levels and protein yield.

3. Purification is performed on a Protein A column. Additional purification steps can be performed if required, for an additional cost.

Please note: expression can subsequently be scaled up to 0.1 - 20 liters to produce starting material at the milligram to gram scale.

1. Sequence of VH and VL
2. DNA template (if DNA synthesis is not desired)
3. Sequence of complete IgG (if specific constant region required)

1. Purified IgG
2. Certificate of Analysis
3. Study Report

 

Please note that plasmid DNA will not be provided if LakePharma's vector system is utilized.