Affinity Measurement

We employ two very powerful platforms to determine the kinetic properties of antibody-antigen interactions:

1.

High throughput (as high as 384 antibodies tested per plate) array SPR by Carterra LSA for full kinetic characterization of antibody-target interactions, also used for antibody fragments expressed in crude periplasmic extracts

2.  

BLI-based Octet system

Please contact LakePharma at inquiries@lakepharma.com for custom quotes and details.

Click here for a case study of epitope binning using Carterra LSA from PEGS 2018 presentation. 

Download Antibody Discovery brochure  Download PEGS Presentation - Integrated Solutions for Antibody Discovery and Beyond  View webinar: Discovery of Potent, Functional mAbs using Hybridoma and Phage Display Platforms Introduction to antibody libraries eBook
Cat.# Service Name Timeline Size Price Quantity Request
24160 Affinity Measurement by Carterra LSA or Octet BLI platforms TBD TBD Request

1. Antibody is captured on the biacore chip or BLI sensor using an appropriate method.

2. Association and dissociation is measured on the Biacore T100 or the ForteBio Octet QKe using varying concentrations of antigen and the kD is calculated using an appropriate curve-fitting mode.

1. Antibody

2. Antigen

Study report