Antibody Affinity Maturation

At LakePharma, different antibody affinity maturation methodologies using phage and yeast surface display platforms followed by high-throughput (HT) screening are employed to increase target binding affinity. 

LakePharma's Affinity Maturation Services:

 

Key Highlights:

CDR randomization of different Ab fragment scaffolds (scFv, Fab, VHH)
•  Large single or combination variable heavy or light chain yeast or phage display CDR libraries can be generated
•  Multiple rounds of panning selections with increasing stringency can be performed in order to achieve demanding affinity improvements
 

Light chain shuffling antibody libraries
•  Yeast or phage display libraries generated via amplification of VL pools from B cells derived from human donors
 

Antibody kinetic characterization using state-of-the-art instrumentation
•  Carterra® LSA array-based SPR for HT kinetic characterization of unpurified bacterial extracts enables early lead prioritization
•  Carterra® LSA or BLI-based Octet used for IgG candidate kinetic characterizations

 

 

Cat.# Service Name Timeline Size Price Quantity Request
24110 Affinity maturation TBD TBD Request