Antibody Affinity Maturation

At LakePharma, different antibody affinity maturation methodologies using phage and yeast surface display platforms followed by high-throughput (HT) screening are employed to increase target binding affinity. 

LakePharma's Affinity Maturation Services:


Key Highlights:

CDR randomization of different Ab fragment scaffolds (scFv, Fab, VHH)
•  Large single or combination variable heavy or light chain yeast or phage display CDR libraries can be generated
•  Multiple rounds of panning selections with increasing stringency can be performed in order to achieve demanding affinity improvements

Light chain shuffling antibody libraries
•  Yeast or phage display libraries generated via amplification of VL pools from B cells derived from human donors

Antibody kinetic characterization using state-of-the-art instrumentation
•  Carterra® LSA array-based SPR for HT kinetic characterization of unpurified bacterial extracts enables early lead prioritization
•  Carterra® LSA or BLI-based Octet used for IgG candidate kinetic characterizations


Download Antibody Discovery brochure  Download PEGS Presentation - Integrated Solutions for Antibody Discovery and Beyond  View webinar: Discovery of Potent, Functional mAbs using Hybridoma and Phage Display Platforms


"LakePharma was a great partner; very helpful, focused, and committed. Thank you for your antibody engineering and production services which made this project a success."

- Maria Day, ImmuNext

LakePharma is proud to contribute to ImmuNext's success.
View their press release here


Cat.# Service Name Timeline Size Price Quantity Request
24110 Affinity maturation TBD TBD Request