Epitope Binning and Mapping

Epitope binning investigates the sequential binding of two antibodies to an antigen. Antibodies with similar blocking profiles are grouped into bins specific to the same or overlapping epitopes. LakePharma uses two proven platforms to test the ability of multiple antibodies to block each other’s binding to the antigen.

1. 

State-of-the-art array-based SPR by Carterra® LSA™ for high throughput (as high as 96 x 96 antibodies tested per plate) epitope binning

2. 

BLI platform-based Octet® system (as high as 16 x 16 antibodies tested)

         

Array-based SPR (Carterra® LSA™)

BLI-based Octet® system

Array-based SPR (Carterra® LSA™)

BLI-based Octet® system

 

Key Highlights

Protocol Customization

LakePharma can customize epitope binning protocols per customer requirements

 

Carterra® LSA™ Instrument

LakePharma is an authorized partner of Carterra®. As the only CRDMO with a Carterra® LSA™ instrument, LakePharma offers a full suite of kinetic assay services including:

 

Kinetics

Epitope Binning

Epitope Mapping

Screen up to 384 antibodies against multiple antigens in a single run using <50 ug of antibody

Test up to 96 antibodies in a pairwise combinatorial manner to group into bins

Interrogate antibody libraries against a 384-peptide array with overlapping sequences to map the epitope

 

Please contact LakePharma at inquiries@lakepharma.com for custom quotes and details.

Download Antibody Discovery brochure  Download PEGS Presentation - Integrated Solutions for Antibody Discovery and Beyond  View webinar: Discovery of Potent, Functional mAbs using Hybridoma and Phage Display Platforms
Cat.# Service Name Timeline Size Price Quantity Request
Custom Epitope Binning and Mapping by Carterra LSA or Octet BLI platforms TBD TBD Request

Antigen is immobilized onto a biolayer interferometry (BLI) sensor using an appropriate method. Antibody 1 is allowed to saturate its epitope on the antigen.

Binding of Antibody 2 to antigen saturated with Antibody 1 is assessed. 

- 100 ug each of purified antibody

- 100 ug of purified antigen

-Study Report