FcRy Binding Assays and FcRn Binding Assays

Measuring an antibody’s affinity to the Fcγ receptors has become an integral part of biotherapeutics development. LakePharma has established a standard human Fcγ receptor panel binding assay in a high throughput format using the BLI platform. This in vitro binding assay can also be used for quality control purposed in parallel with functional cell-based ADCC. Additionally, LakePharma offers clients an FcRn binding assay to study pH-dependent interaction of antibody with the neonatal Fc-receptor (FcRn) in high throughput and easy to use format. The outcome of the study will enhance overall understanding of the antibody and demonstrate comparability in the development of biosimilars. 

Please contact LakePharma at inquiries@lakepharma.com for custom quotes and details.

Click here for more information on cell-based antibody characterization assays.

Cat.# Service Name Timeline Size Price Quantity Request
Fcγ receptor panel binding assay 1-2 weeks TBD $3500 per antibody

The panel is performed using Octet Red96 (ForteBio):

- his-tagged receptors are immobilized onto anti-His biosensors

- the loaded biosensors are dipped into a dilution series of the antibody

- the on- (Kon) and off-rates (Koff) are measured and the KD value is calculated

100 µg of purified antibody.

Study Report.

Please click here to view a case study in PDF format.