Primary T-Cell Functional Assays

T-cell based immunotherapy strategies have become increasingly important in disease treatments that harness the human immune system. LakePharma offers a variety of assays for characterizing T-cell activity with candidate antibodies.

    1. T-cell activation and proliferation assays include stimulation with staphylococcal                           enterotoxin B (SEB), cytomegalovirus (CMV) recall antigen, and anti-CD3 antibodies.

    2. Assay readouts include cytokine measurement by ELISA or FACS.

    3. Mixed lymphocyte reaction (MLR) and T-regulatory suppression assays.

Please contact LakePharma at inquiries@lakepharma.com for custom quotes and details.

View LakePharma T-Cell Functional Assay Case Studies.

Cat.# Service Name Timeline Size Price Quantity Request
Custom T-cell Stimulation Assay 3-4 weeks per 96-well plate Request
Custom T-cell Proliferation Assay 3-4 weeks per 96-well plate Request
Custom CMV Recall Assay 3-4 weeks per 96-well plate Request
Custom Mixed Lymphocyte Reaction 3-4 weeks per 96-well plate Request
Custom T-Reg Suppression Assay 2-3 weeks per 96-well plate Request

T-CELL STIMULATION ASSAY

1. PBMCs, SEB, and test antibodies are co-incubated for 3 days

2. IL-2 concentrations measured by ELISA or FACS

 

T-CELL PROLIFERATION ASSAY

1. PBMCs, anti-CD3 antibodies (can be plate bound, soluble, or anti-CD3 coated beads), test antibodies, and CSFE dye are co-incubated for 3 days

2. Samples harvest and stained with anti-CD4

3. T-cell proliferation assessed by FACS

 

CMV RECALL ASSAY

1. Incubate PBMCs from CMV-positive donors with CMV pp65 peptide pool and test antibodies for 3 days.

2. IFN-γ ELISA performed on collected supernatants.

 

MIXED LYMPHOCYTE REACTION

1. Macrophage and dendritic cell generation from two individual donors and FACS validation.

2. Co­culture at single ratio with allogeneic frozen PBMCs from 2 individual donors (4 pairwise combinations + controls)

3. Add test and control antibodies.

4. IL-2 ELISA performed

 

T-REG SUPPRESSION ASSAY

1. PBMCs isolated, T-reg population sorted.

2. Unsorted PBMCs labeled with CFSE

3. Co-incubation with T-reg cells, PBMCs, test antibodies for 4 days

4. T-reg suppression measured by FACS

 

 

Test antibodies

Studay report