Reporter Gene and Immune Checkpoint Assays

LakePharma offers a wide variety of custom functional assays to test candidate antibodies involved in various pathways:  

    1. Immune checkpoint blockade assays from PD-1/PD-L1, CTLA-4, and GITR pathways.               Readout by FACS or ELISA.

    2. Custom cell-based, reporter-gene, or receptor-ligand assays.

    3. Performed as transfer of an existing assay or developed as fully custom workflow.

Please contact LakePharma at inquiries@lakepharma.com for custom quotes and details.

View LakePharma PD-1/PD-L1 Immunoblockade Reporter Assay Case Study.

Cat.# Service Name Timeline Size Price Quantity Request
Custom Custom Reporter Gene Assay 2-3 weeks per 96-well plate Request
Custom Custom Cell-Based Assay 2-3 weeks per 96-well plate Request
Custom Immune Checkpoint Bioassays 2-3 weeks per 96-well plate Request
Custom Receptor-Ligand Blocking Assay 2-3 weeks per 96-well plate Request
Custom Antibody-Mediated Viral Neutralization Assay 2-3 weeks per 96-well plate Request

PD-1/PD-L1 IMMUNOBLOCKADE REPORTER ASSAY

1. Culture effector cells stably expressing ligand with luciferase-induced intracellular signaling factor

2. Co-culture effector cells with target cells stably expressing target receptor and test antibodies

3. Determine luminescence and EC50

Test antibodies

Study report