Catalog #1133 - Molecular construction of IgG, 0.03 L transient production in HEK293 cells (Tuna293™ standard 7-day process), Protein A purification (template provided)

Title:
Molecular construction of IgG, 0.03 L transient production in HEK293 cells (Tuna293™ standard 7-day process), Protein A purification (template provided)

Description:

Molecular construction of IgG in LakePharma's proprietary expression vector, 0.03 L transient production in HEK293 cells (Tuna293™ process), Protein A purification (template provided)

- Includes heavy + light chain

- Includes DNA scale-up and DNA sequence confirmation

- CE-SDS analysis QC is included

- Intact mass QC by mass spectrometry is included


Scope:

Molecular construction only services:

Antibody genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

Scale-up productions are available at an additional cost


Packages for molecular construction and small scale production:

1. Antibody genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

2. Pilot small-scale (0.03 liter) transient transfection into HEK293 cells (Tuna293™ Process) is performed, followed by the listed purification method to assess expression levels and protein yield. Or

3. Pilot small-scale (0.03 liter) transient transfection into CHOcells (TunaCHO™ Process) is performed, followed by the listed purification method to assess expression levels and protein yield.


Deliverable:

Molecular construction only services:

-plasmid DNA (if in a commercially available vector)

OR

-plasmid DNA used in scale-up productions at LakePharma


Packages for molecular construction and small scale production:

1. Purified IgG

2. Study report

3. Certificate of Analysis


Please note that plasmid DNA will not be provided if LakePharma's vector system is utilized.


Material Required:

1. Sequence of VH and VL

2. DNA template (if DNA synthesis is not desired)

3. Sequence of complete IgG (if specific constant region required)


Timeline:
4 weeks

Price:
$2,480