HEK293 Transient Antibody Production (Tuna293™ Process)

For recombinant antibody production at scales ranging from hundreds of micrograms to hundreds of milligrams, expression constructs are transiently transfected into HEK293 cells at various scales followed by protein purification. The services listed below use our in-house Tuna293™ Process, generating higher yields for a smaller volume production.

New - Intact mass spec analysis for all recombinant antibody productions. 

Cat.# Service Name Timeline Size Price Quantity Request
27625 0.03 liter transient production and protein A purification* 2-3 weeks 0.03 liter $1000
27630 0.1 liter transient production and protein A purification* 2-3 weeks 0.1 liter $1650
27645 0.5 liter transient production and protein A purification* 2-3 weeks 0.5 liter $4125
27655 2 liter transient production and protein A purification* 2-3 weeks 2 liter $7700
27670 10 liter transient production and protein A purification* 2-3 weeks 10 liter $15750
28510 Buffer exchange following purification (up to 250 mL working volume) 3 - 5 days up to 250 mL $300
Note: Antibody Scale-Up Production Services. Purification using Protein G is available- please inquire: inquires@lakepharma.com *all services listed do not include DNA scale-up; additional costs may apply. Additional scale-up volumes are available. Please inquire: inquiries@lakepharma.com

1.HEK293 cells are scaled up to 0.03 - 10 liter for Tuna293 transient transfection to produce material at the milligram to gram scale

2.The listed purification method will be used to obtain purified antibody

3. DNA scale up cost is not included

1. Sequence of antibody

2. Transfection ready DNA (inquire if DNA scale-up is needed)

1. Purified protein

2. Study Report

3. Certificate of Analysis