Starting from gene synthesis, 96 antibodies will be constructed, produced, purified, and delivered in 5 to 7 weeks.
View PDF file on TunaCHO for high yield transient antibody and protein production.
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Molecular construction of IgG expression constructs in LakePharma’s proprietary vector (synthesize variable regions)
DNA scale-ups (midiprep scale), including sequence confirmation
0.01 liter transient productions in HEK293 cells (HTP Tuna293™ Process) or CHO cells (HTP TunaCHO™ Process)
HTP Protein A purifications
Intact Mass Spec (reducing and deglycosylated) QC
1. VH and VL sequences for 96 antibodies.
2. DNA template (if DNA synthesis is not desired)
3. Specified constant region for all 96 antibodies. Additioinal fees may apply for more than one heavy chain and one light chain.
Please note: one constant region must be specified for all 96 antibodies.
All purified protein in HEPES (100 mM, 100 mM NaCl, 50 mM NaOAc, pH 6.0) and unadjusted concentration obtained from elution, frozen in 96-well plate
Certificate of Analysis including summary of yields and aliquots
Mass Spec match/flag report
Additional service options:
Sterile filtration: $1000/plate
Individual aliquots: $1200/plate