High Throughput 96-Block Antibody Production

The demand for high throughput antibodies has exponentially increased over the last couple of years and has rapidly accelerated the antibody screening process. LakePharma offers 96-well antibody production service which is a rapid and cost-effective process to produce large quantities of antibodies for fast antigen-binding assay and productivity screening. 

 

Workflow of High Throughput 96-Block Antibody Production

Gene synthesis


Key Highlights

 

Large Quantities of Antibody Production

•  TunaCHO™ and Tuna293™ processes with 10 mL to 100 mL production scale

 

 

Rapid Production

•  Quick turnaround time between 3 to 7 weeks based on starting material

•  Complementary mass spec QC for standard mouse and human IgG antibody

 

 Download Antibody Discovery brochure  View webinar: Discovery of Potent, Functional mAbs using Hybridoma and Phage Display Platforms
       

 

Please contact LakePharma at inquiries@lakepharma.com for custom quotes and details.

Cat.# Service Name Timeline Size Price Quantity Request
27680 96-batch molecular construction of IgG expression vectors, 0.01 L transient production in HEK293 cells (Tuna293™ Process), Protein A purification 5-6 weeks 96 $96,000
96-batch molecular construction of IgG expression vectors, 0.01 L transient production in CHO cells (TunaCHO™ 7 Days Process), Protein A purification 5-6 weeks 96 $96,000
28680 96-batch molecular construction of IgG expression vectors, 0.01 L transient production in CHO cells (TunaCHO™ Process, 14-day extended production), Protein A purification 6-7 weeks 96 $115,200
  1. Molecular construction of IgG expression constructs in LakePharma’s proprietary vector (synthesize variable regions)

  2. DNA scale-ups (midiprep scale), including sequence confirmation

  3. 0.01 liter transient productions in HEK293 cells (HTP Tuna293™ Process) or CHO cells (HTP TunaCHO™ Process)

  4. HTP Protein A purifications

  5. Intact Mass Spec (reducing and deglycosylated) QC

 

1. VH and VL sequences for 96 antibodies.

2. DNA template (if DNA synthesis is not desired)

3. Specified constant region for all 96 antibodies. Additioinal fees may apply for more than one heavy chain and one light chain.

 

Please note: one constant region must be specified for all 96 antibodies.

 

  1. All purified protein in HEPES (100 mM, 100 mM NaCl, 50 mM NaOAc, pH 6.0) and unadjusted concentration obtained from elution, frozen in 96-well plate

  2. Study Report

  3. Certificate of Analysis including summary of yields and aliquots

  4. Mass Spec match/flag report

 

Additional service options:  

  1. Sterile filtration: $1000/plate

  2. Individual aliquots: $1200/plate