Molecular Construction and Small Scale Antibody Production

LakePharma provides plasmid design and construction services for the production of recombinant antibodies. LakePharma’s in-house proprietary vector system ensures compatibility with Tuna293™ Process or TunaCHO™ Process for transient antibody production in HEK293 or CHO cells.

Multiple species and isotypes are available, with the option of synthesizing a custom constant region.

For TunaCHO, choose standard 7-day production for quick production cycle or 14-day extended production for higher yield.  Click here to learn more about TunaCHO performance.

Please contact LakePharma at inquiries@lakepharma.com for custom quotes and details.

Cat.# Service Name Timeline Size Price Quantity Request
1133 Molecular construction of IgG expression vectors, 0.03 liter transient production in HEK293 cells (Tuna293™ Process), and protein A purification (template DNA provided by client) 4-5 weeks 0.03 liter $2480
1134 Molecular construction of IgG expression vectors, 0.03 liter transient production in HEK293 cells (Tuna293™ Process) and protein A purification (synthesize variable regions) 4-5 weeks 0.03 liter $2980
1794 Molecular construction of IgG expression vectors, 0.03 liter transient production in CHO cells (TunaCHO™ Process, standard 7-day production), protein A purification (template DNA provided by client) 4-5 weeks 0.03 liter $2580
1795 Molecular construction of IgG expression vectors, 0.03 liter transient production in CHO cells (TunaCHO™ Process, standard 7-day production) and protein A purification (synthesize variable regions) 4-5 weeks 0.03 liter $3080
1796 Molecular construction of IgG expression vectors, 0.03 liter transient production in CHO cells (TunaCHO™ Process, extended 14-day production), protein A purification (template DNA provided by client) 5-6 weeks 0.03 liter $3150
1797 Molecular construction of IgG expression vectors, 0.03 liter transient production in CHO cells (TunaCHO™ Process, extended 14-day production), protein A purification (synthesize variable regions) 5-6 weeks 0.03 liter $3650

Molecular construction only services:

Antibody genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

Scale-up productions are available at an additional cost

 

Packages for molecular construction and small scale production:

1. Antibody genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

2. Pilot small-scale (0.03 liter) transient transfection into HEK293 cells (Tuna293™ Process) is performed, followed by the listed purification method to assess expression levels and protein yield. Or

3. Pilot small-scale (0.03 liter) transient transfection into CHOcells (TunaCHO™ Process) is performed, followed by the listed purification method to assess expression levels and protein yield.

1. Sequence of VH and VL

2. DNA template (if DNA synthesis is not desired)

3. Sequence of complete IgG (if specific constant region required)

Molecular construction only services:

-plasmid DNA (if in a commercially available vector)

OR

-plasmid DNA used in scale-up productions at LakePharma

 

Packages for molecular construction and small scale production:

1. Purified IgG

2. Study report

3. Certificate of Analysis

 

Please note that plasmid DNA will not be provided if LakePharma's vector system is utilized.