Molecular Construction and Small Scale Antibody Production

LakePharma provides plasmid design and construction services for the production of recombinant antibodies. LakePharma’s in-house proprietary vector system ensures compatibility with Tuna293™ Process or TunaCHO™ Process for transient antibody production in HEK293 or CHO cells.

For antibodies, multiple species and isotypes are available, with the option of synthesizing a custom constant region.

Click here to learn more about TunaCHO for high yield antibody production.

New - Intact mass spec analysis for all recombinant antibody productions. 

Cat.# Service Name Timeline Size Price Quantity Request
27600 Molecular construction of IgG expression vectors, 0.03 liter transient production in HEK293 cells (Tuna293™ Process), and protein A purification (template DNA provided by client) 4-5 weeks 0.03 liter $2400
27610 Molecular construction of IgG expression vectors, 0.03 liter transient production in HEK293 cells (Tuna293™ Process) and protein A purification (synthesize variable regions) 4-5 weeks 0.03 liter $2900
28600 Molecular construction of IgG expression vectors, 0.05 liter transient production in CHO cells (TunaCHO™ Process), and protein A purification (template DNA provided by client) 5-6 weeks 0.05 liter $3250
28610 Molecular construction of IgG expression vectors, 0.05 liter transient production in CHO cells (TunaCHO™ Process) and protein A purification (synthesize variable regions) 5-6 weeks 0.05 liter $3750

Molecular construction only services:

Antibody genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

Scale-up productions are available at an additional cost

 

Packages for molecular construction and small scale production:

1. Antibody genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

2. Pilot small-scale (0.03 liter) transient transfection into HEK293 cells (Tuna293™ Process) is performed, followed by the listed purification method to assess expression levels and protein yield. Or

3. Pilot small-scale (0.05 liter) transient transfection into CHOcells (TunaCHO™ Process) is performed, followed by the listed purification method to assess expression levels and protein yield.

1. Sequence of VH and VL

2. DNA template (if DNA synthesis is not desired)

3. Sequence of complete IgG (if specific constant region required)

Molecular construction only services:

-plasmid DNA (if in a commercially available vector)

OR

-plasmid DNA used in scale-up productions at LakePharma

 

Packages for molecular construction and small scale production:

1. Purified IgG

2. Study report

3. Certificate of Analysis

 

Please note that plasmid DNA will not be provided if LakePharma's vector system is utilized.