We employ two methods for glycan analysis: a capillary electrophoresis (CE-) based method and a mass spectrometry based method. If you want to know the absolute quantification of common glycosylations, we recommend the CE-based method. If you want to know all types of glycosylation and their locations, we recommend mass spectrometry.
1. Multiple methods to analyze the N-glycan profile
2. Experienced scientist help to choose the right method
3. Proficient Interpretion to ensure your product quality
Please contact firstname.lastname@example.org for custom quotes and details.
|Note: a $500 setup fee may be applied for projects with <10 samples.|
1. Samples are deglycosylated using a PNGase F treatment
2. Released glycans are fluorescently labelled
3. Separation by microchip based CE-SDS
4. Observed peaks are compared against a set of N-glycan standards (Man5, G0, G0F, G1, G1`, G1F, G1`F, G2, G2F) for identification.
1. The antibody/protein of interest is denatured, reduced, and digested with one or more proteolytic enzymes, such as trypsin.
2. The resulting peptide mixtures are submitted to a reverse-phase HPLC coupled to a mass spectrometer.
3. The mass spectra is compared against the target protein sequence and the glycans of interests are identified (if present).
Note: the above quoted prices are based on analysis using LakePharma’s standardized methods, proven to work for a majority of antibodies and proteins. Method development may be necessary for atypical proteins with unique properties for an additional fee.
1. 100 ug of purified protein at ≥1 mg/mL
2. Target protein sequence (Catalog 63120)
Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to LakePharma for analysis