N-terminal Sequencing by Edman Sequencing

N-terminal Edman sequencing of proteins or peptides determines the N-terminal amino acid residues of purified protein and antibody samples. It can be useful to determine cleavage sites, to identify an unknown protein, to confirm the identity of a known protein, or to design oligonucleotide probes. Advantages of N-terminal sequencing include high sensitivity, with measurements on as small a scale as picomoles. Proteins can also be sequenced in solution or on PVDF blots.

Key benefits:

  1. High sequence coverage - Identify up to 15 amino acids

  2. Fast turnaround: as fast as 3 days

  3. High sensitivity – sample size as small as picomoles 

Please contact inquiries@lakepharma.com for custom quotes and details.

Cat.# Service Name Timeline Size Price Quantity Request
64005 N-terminal sequencing (5 residue) 1 week per sample $350
64010 N-terminal sequencing (10 residue) 1 week per sample $450
64020 N-terminal sequencing (15 residue) 1 week per sample $550
Note: a $500 setup fee may be applied for projects with <10 samples.

1. The protein sample is denatured and reduced prior to running on an SDS-PAGE gel.

2. The sample is transferred to a PVDF membrane using a wet transfer protocol.

3. The band(s) of interest is excised and submitted to an ABI 494 Protein Sequencer.

4. In the event the N-terminus of the protein is blocked and no sequences are obtained, the full project price is still invoiced.

 

Note: the above quoted prices are based on analysis using LakePharma’s standardized methods, proven to work for a majority of antibodies and proteins. 

1. 100 ug of purified protein at ≥1 mg/mL

2. (Optional) Target protein sequence

3. (Optional) Molecular weight of expected bands

 

Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to LakePharma for analysis

Study report with N-terminal amino acid sequences of purified protein samples