N-terminal Sequencing by Edman Sequencing

N-terminal Edman sequencing of proteins or peptides determines the N-terminal amino acid residues of purified protein and antibody samples. It can be useful to determine cleavage sites, to identify an unknown protein, to confirm the identity of a known protein, or to design oligonucleotide probes. The advantages of N-terminal sequencing include high sensitivity, with measurements on as small a scale as picomoles. Proteins can also be sequenced in solution or on PVDF blots.

Key benefits

1.

High sequence coverage - Identify up to 15 amino acids

2.

Fast turnaround: as fast as 3 days

3.

High sensitivity – sample size as small as picomoles 

 

​Please contact inquiries@lakepharma.com for custom quotes and details.

Cat.# Service Name Timeline Size Price Quantity Request
64000 SDS-PAGE with transfer to PVDF and Ponseau S staining 1 week per 4 samples $1000
64005 N-terminal sequencing (5 residue) 1 week per sample $400
64010 N-terminal sequencing (10 residue) 1 week per sample $500
64020 N-terminal sequencing (15 residue) 1 week per sample $600
Note: a $500 setup fee may be applied to any bioanalytical assay containing <10 samples.

Cat.# 64000:

  1. The protein sample is denatured and reduced prior to running on an SDS-PAGE gel.
  2. The sample is transferred to a PVDF membrane using a wet transfer protocol.

Cat.# 64005, 64010, 64020:

  1. The band(s) of interest is excised and submitted to an ABI 494 Protein Sequencer.
  2. In the event, the N-terminus of the protein is blocked and no sequences are obtained, the full project price is still invoiced.

 

Note: the above-quoted prices are based on analysis using LakePharma’s standardized methods, proven to work for a majority of antibodies and proteins.

Cat.# 64000:

  1. 100 µL at > 0.5 mg/mL
  2. The molecular weight of the target band(s) if paired with Cat.# 64005, 64010, 64020

Cat.# 64005, 64010, 64020:

  1. PVDF membrane (Coomassie Blue or Ponceau S stained) cutout of target bands

 

Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to LakePharma for analysis.

Cat.# 64000:

  • PVDF membrane with stained protein band(s)

Cat.# 64005, 64010, 64020:

  • Edman degradation cycle chromatogram with calls for each cycle