N-terminal Edman sequencing of proteins or peptides determines the N-terminal amino acid residues of purified protein and antibody samples. It can be useful to determine cleavage sites, to identify an unknown protein, to confirm the identity of a known protein, or to design oligonucleotide probes. Advantages of N-terminal sequencing include high sensitivity, with measurements on as small a scale as picomoles. Proteins can also be sequenced in solution or on PVDF blots.
High sequence coverage - Identify up to 15 amino acids
Fast turnaround: as fast as 3 days
High sensitivity – sample size as small as picomoles
Please contact firstname.lastname@example.org for custom quotes and details.
|Note: a $500 setup fee may be applied for projects with <10 samples.|
1. The protein sample is denatured and reduced prior to running on an SDS-PAGE gel.
2. The sample is transferred to a PVDF membrane using a wet transfer protocol.
3. The band(s) of interest is excised and submitted to an ABI 494 Protein Sequencer.
4. In the event the N-terminus of the protein is blocked and no sequences are obtained, the full project price is still invoiced.
Note: the above quoted prices are based on analysis using LakePharma’s standardized methods, proven to work for a majority of antibodies and proteins.
1. 100 ug of purified protein at ≥1 mg/mL
2. (Optional) Target protein sequence
3. (Optional) Molecular weight of expected bands
Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to LakePharma for analysis
Study report with N-terminal amino acid sequences of purified protein samples