Sequence Confirmation by LC-MSMS

At LakePharma, our highly-experienced staff have developed a proprietary methodology which gives consistent results over the years. Combining with our high resolution mass spectrometry, we typically achieve more than 95% sequence coverage with single enzyme digestion; and can reach to 98% - 100% with two or more enzyme digestion.

Key benefits:

  1. Proprietary methodology providing consistent results

  2. Highly experienced in analyzing various types of molecules

  3. Super high resolution mass spectrometry ensures highest quality results

  4. 95% coverage with single enzyme digestion; 98%-100% coverage with two or more enzymes

 

Please contact inquiries@lakepharma.com for custom quotes and details.

Cat.# Service Name Timeline Size Price Quantity Request
61200 Sequence Confirmation by 1 Enzyme Digestion 1-2 weeks each sample $700
61220 Sequence Confirmation by 2 Enzyme Digestion 1-2 weeks each sample $900
61225 Custom Sequence Confirmation 1-2 weeks each sample Request
Note: a $500 setup fee may be applied for projects with <10 samples.

1. The antibody/protein of interest is denatured, reduced and digested with one or more proteolytic enzymes, such as trypsin.

2. The resulting peptide mixtures are separated on a reverse-phase HPLC coupled to a mass spectrometer

3. The mass spectra is compared against the target protein sequence and the percent coverage is calculated.

 

Note: the above quoted prices are based on analysis using LakePharma’s standardized methods, proven to work for a majority of antibodies and proteins. Method development may be necessary for atypical proteins with unique properties for an additional fee.

1. 100 ug of purified protein at ≥1 mg/mL

2. Target protein sequence

3. (Optional) 10 mL blank buffer formulation

 

Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to LakePharma for analysis

Study report