Thermostability by DSC or DSF

Our highly-experienced scientists can help you choose the right conditions to obtain accurate thermostability analysis. In addition to the industry standard differential scanning calorimetry (DSC), you can also choose the newest differential scanning fluorescence technology (DSF), which utilizes intrinsic fluorescence over fluorescent dye.

Key benefits:

     1. Determine the melting temperature and thermostability

     2. Compare the native and mutant variants

     3. Rapid assessment of large sample batches 

     4. Small sample size over broad concentration range

     5. Perform particle size and aggregation analysis in parallel 


Contact for your custom quotes.

Cat.# Service Name Timeline Size Price Quantity Request
62200 Thermostability Analysis by DSC 1-2 weeks Each sample $600
62210 Thermostability Analysis by DSF 4-5 days Each sample $400
Note: a $500 setup fee may be applied to any bioanalytical assay containing <10 samples.

1. The antibody/protein sample is injected into either the Nano DSC instrument (TA instrument) or UNcle (Unchained Labs).

2. The Tm (melting temperature where 50% protein is denatured) is calculated after deconvolution using the analytical software

Note: the above quoted prices are based on analysis using LakePharma’s standardized methods, proven to work for a majority of antibodies and proteins. Method development may be necessary for atypical proteins with unique properties for an additional fee.

Catalog 62200 (DSC):

1. 1 mg of purified protein at ≥1 mg/mL

2. 10 mL blank buffer formulation


Catalog Custom (DSF):

1. 100 ug of purified protein at ≥1 mg/mL

2. (Optional) 10 mL blank buffer formulation


Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to LakePharma for analysis

Report containing heat stability data for test articles as measured by Tm (thermal transition mid-point)