Biochemical Assays

Curia performs a wide variety of biochemical assays to support drug discovery efforts in both the small and large molecule arenas. Our Assay team has a broad experience base traditional enzyme kinetic analyses and ligand-binding assays as well as cutting edge kinetic analysis utilizing Octet Biolayer Interferometry (BLI) utilizing a wide variety of readouts and technologies. The assay team leverages the full menu of Multimode Readers, Genetic Assay Technologies, and Analytical Instruments available across Curia. A variety of liquid handling technologies and platforms are available to support studies at a small scale as well as at full HTS scale.

Assay Type Highlights:

• Enzyme Activity and Kinetic Evaluation
• Binding Affinity Determinations
• Immunoassays
• Membrane Prep Ligand-binding Assays
• HTS/Structure activity relation (SAR) screening and Secondary screen potency determinations
• Full customization of workflow and assay formats available

Assay Format/Technology Highlights:

• Fluorescent/Luminescence: HTRF, FP, FRET, AlphaLISA, AlphaScreen, Glo, Luciferase
• ELISA-Based: Meso Scale Discovery ECL and traditional TMB
• Spectrophotometric Abs and Radiometric Readouts
• HPLC and LC/MS methodologies
• Full Custom Development; Discovery through cGxP Assay Support

Liquid Handling Highlights:

• Formulatrix Tempest Automated Dispenser Platform
• 96/384 Channel Pipettors from Integra Biosciences and Apricot Technologies
• Tecan d300e Digital Dispenser
• Labcyte Echo 550 (3rd Party Collaboration)