Catalog #1914

Title:
Megaprep scale Transfection grade (estimated <100 EU/mg) DNA production service (estimated yield of 2.0-4.0 mg)

Description:
Megaprep scale Transfection grade (estimated <100 EU/mg) DNA production service (estimated yield of 2.0-4.0 mg)

Scope:

1. Client-provided plasmid DNA is transformed into competent cells.

2. Cells are cultured up to the desired scale.

3. Plasmid DNA is purified and processed with appropriate endotoxin removal steps.

4. Additional services for low endotoxin requirement (<10 EU/mg, <1 EU/mg), plasmid sequence verification, and/or restriction enzyme digestion are available for additional fee. 


Note: Estimated yields based on typical production methods using LakePharma vectors. Actual yields may vary depending on the number of copies of the supplied vector. Purified plasmid DNA through the endotoxin-free process is typically less than 100 EU/mg. Please contact us for more information.


Deliverable:
Purified plasmid with Certificate of Analysis and agarose gel analysis.

Material Required:

1. Either plasmid DNA (1-2 ug), or a plate with bacterial colonies.

2. Information on antibiotic resistance of the plasmid.
3. Information for sequence or restriction enzyme verification, if confirmation is needed (additional charges may apply).


Note: Please complete and include a sample submission form with the samples you send to LakePharma.


Disclaimer: We highly recommend working with Ampicillin/Carbenicillin or Kanamycin resistance for bacterial scale ups. Any other bacterial selection marker may require an extended timeline and/or extra charge.


Timeline:
5-7 Business Days

Price:
$450