HRP Conjugation

Conjugated antibodies are used in a variety of laboratory techniques, including ELISA immunoassays and Western blots, as a means of signal generation. LakePharma uses proprietary conjugation techniques to achieve maximum stability and conjugation yield.

 

The enzyme horseradish peroxidase (HRP) can be attached to your protein or antibody to create signal-generating molecules. Sugar groups on native HRP are oxidized with periodate to produce amine-reactive aldehydes. The activated peroxidase spontaneously and efficiently crosslinks with primary amines on the antibody or other proteins. This method directs the coupling location to the Fc portion of the antibody molecule and is more effective than other amine-reactive chemistries, such as glutaraldehyde coupling, which often causes polymerization and conjugate inactivation.

Cat.# Service Name Timeline Size Price Quantity Request
28101 HRP Conjugation 2 weeks 2-10mg $1300

Standard conjugation reaction followed by purification.

Purified proteins and antibodies (>2 mg)

1. HRP-conjugated proteins and antibodies

2. Certificate of Analysis

 

Please note: the final delivered amount may be less than the amount of starting material initially provided.