Biotinylation

Biotinylation, the process of covalently attaching biotin, can be performed on your protein or antibody. Because of its small size, biotin is a popular conjugate that has little effect on an antibody's immunoreactivity. Biotin can bind to streptavidin and avidin with high affinity and is used to increase the sensitivity of detection in many immunological procedures.

Cat.# Service Name Timeline Size Price Quantity Request
28105 Biotinylation 1 week < 5 mg proteins or antibodies $1300
Custom Biotinylation 1 week 5 - 20 mg proteins or antibodies $2000
Custom Biotinylation 1 week 21 - 50 mg proteins or antibodies $3000
  1. Biotinylation of client provided proteins targeting a client specified Biotin-to-Protein ratio
  2. Excess biotin removal using dialysis
  3. Buffer exchange into PBS; all other formulations may require an additional fee per sample
  4. Quantitation of the average number of biotins per protein
  5. Low endotoxin processing available for additional costs

Purified proteins for labeling

  1. All biotinylated proteins
  2. Buffer formulation: PBS; all other formulations may require an additional fee per sample
  3. Protein concentration: Client specified
  4. Endotoxin levels: < 1 EU/mg if low endotoxin service is requested
  5. COA includes: Final protein concentration, average number of biotins per protein ratio, final buffer composition, endotoxin levels
  6. Standard aliquoting (up to 5 vials of equal volume, with the remainder in glass vials); Custom aliquoting available for additional costs, please see below
  7. Protein shipping temperature: -80°C