Catalog #1049 - Biotinylation of Avi Tag

Title:
Biotinylation of Avi Tag

Description:

< 5 mg site specific in-vitro biotinylation of Avi Tag using BirA

- Standard in-vitro biotinylation labeling

- QC biotin-to-protein ratio

*Additional services required for BirA enzyme removal


Scope:
  1. Biotinylation of client provided proteins targeting a client specified Biotin-to-Protein ratio
  2. Excess biotin removal using dialysis
  3. Buffer exchange into PBS; all other formulations may require an additional fee per sample
  4. Quantitation of the average number of biotins per protein
  5. Low endotoxin processing available for additional costs

Deliverable:
1. All purified biotinylated protein 2. Study report 3. Certificate of Analysis

Material Required:
1. Purified AviTag fused protein to be biotinylated in vitro. OR 2. Gene of interest to be produced with an AviTag.

Timeline:
7 days

Price:
$3,410