Catalog #28106 - Biotinylation of 5-20 mg protein or antibodies

Title:
Biotinylation of 5-20 mg protein or antibodies

Description:

Biotinylation of  5-20 mg protein or antibodies


- [Amount of protein] of [Protein name] will be used for labeling

- Target Biotin-to-Protein ratio of  [Desired B/P ratio]

- Standard biotinylation service

- QC biotin-to-protein ratio

- Endotoxin requirement: [State the endotoxin requirement]


Scope:


  1. Biotinylation of client provided proteins targeting a client specified Biotin-to-Protein ratio
  2. Excess biotin removal using dialysis
  3. Buffer exchange into PBS; all other formulations may require an additional fee per sample
  4. Quantitation of the average number of biotins per protein
  5. Low endotoxin processing available for additional costs

Deliverable:

1. All biotinylated proteins

2. Buffer formulation: PBS; all other formulations may require an additional fee per sample.

3. Protein concentration: Client specified/ N/A

4. Endotoxin levels: < 1 EU/mg if low endotoxin service is requested.

5. COA includes: Final protein concentration, average number of biotins per protein ratio, final buffer composition, endotoxin levels.

6. Standard aliquoting (up to 5 vials of equal volume, with the remainder in glass vials); Custom aliquoting available for additional costs.

7. Protein shipping temperature: -80°C.


Material Required:
Purified proteins for labeling

Timeline:
1-2 week

Price:
$4,180