Catalog #28301 - Fluorophore Labeling of < 5 mg protein or antibodies

Title:
Fluorophore Labeling of < 5 mg protein or antibodies

Description:

Fluorophore Labeling of  < 5 mg protein or antibodies


- [Amount of protein] of [Protein name] will be used for labeling

- [Name of Fluorophore] is the selected fluorophore

- Target Fluorophore-to-Protein ratio (F/P) of  [Desired F/P ratio]

- Standard fluorophore labeling service

- QC F/P ratio using spectrophotometry

- Endotoxin requirement: [State the endotoxin requirement]


*Fluorophore price not included


Scope:
  1. Protein labeling with commercially available dyes derivatized with Succinimidyl-Esters or Maleimides targeting custom Fluorophore to Protein ratios (F/P)
  2. Excess dye removal using dialysis
  3. Buffer exchange into PBS; all other formulations may require an additional fee per sample
  4. F:P determination using spectrophotometry
  5. Low endotoxin processing is available for additional costs

Deliverable:

1. All labeled proteins.

2. Buffer formulation: PBS; all other formulations may require an additional fee per sample.

3. Protein concentration: Client specified/ not applicable

4. Endotoxin levels:

5. COA includes: Final protein concentration, average number of fluorophores per protein ratio, final buffer composition, endotoxin levels if requested.

6. Standard aliquoting (up to 5 vials of equal volume, with the remainder in glass vials)

Custom aliquoting available for additional costs.

7. Protein shipping temperature: -80°C


Material Required:

- Purified proteins for labeling 

- Fluorophores for labeling (A $60 procurement fee will be applied if LakePharma places the order for the reagents)


Timeline:
7 days

Price:
$1,650