- Molecular Biology
- Viral Vectors & Cell Engineering
- Protein Engineering & Production
- Antibody Discovery & Engineering
- Antibody Production
-
Analytics & Formulation
- Overview
- Purity Analysis by CE-SDS
- Aggregation Analysis
- Charge Variant Analysis
- De Novo Antibody Sequencing
- Intact Mass Analysis
- Sequence Confirmation by Mass Spectrometry
- N-terminal Sequencing
- Post-Translational Modification
- N-glycan Analysis
- Thermostability Analysis
- Stability Analysis
- Formulation Development
- Developability Analysis
- R&D and cGxP Bioassay Development
- Protein Chemistry
- Development & Manufacturing in LakePharma Hayward, CA
- Development & Manufacturing in LakePharma Hopkinton, MA
- Consulting Services
Catalog #2003
Title:
In Vitro Glycoengineering Scale Up
Description:
In Vitro Glycoengineering Scale Up
Scope:
- Original sample will be aliquoted for glycan profiling and characterization assays.
- The sample will undergo two steps of the reaction, galactosylation, and sialylation.
- Protein-to-enzyme ratios are determined within the "IVGE Process Optimization" step.
- Samples will be polished using an appropriate purification method.
- Samples after reaction will undergo glycan profiling and characterization assays for comparison.
Deliverable:
- Glycan Profiles- Aggregation analysis report- Purity analysis report- Charge Variant Analysis report
Material Required:
- Client requested amount of purified protein at ≥ 2 mg/mL
- Sequence of target protein
- Theoretical pI value
- Purification method/tag on protein of interest