Mammalian Expression
Starting from plasmid and construction design services, LakePharma provides a variety of protein production and purification services using both CHO and HEK293 cell lines. Affinity tags such as Fc, albumin, poly-histidine (His), maltose-binding protein (MBP), small ubiquitin-related modifier (SUMO), and FLAG are commonly used to facilitate protein purification. Highly streamlined molecular construction and transient production provide a fast turn around time.
Workflow of Mammalian Transient Expression
LakePharma:
1. Provides customized services for the purification of tagless proteins. A combination of ion-exchange, hydrophobic interaction, and other chromatography methods are used to enrich and purify the target molecule from the starting material. See here for more details about custom protein purification.
2. Has an in-house proprietary vector system that ensures compatibility with Tuna293™ Process or TunaCHO™ Process for transient production at various scales in HEK293 or CHO cells.
3. Offers a full line of protein analytical and characterization services. Click here for more details.
4. Also provides stable cell line development for proteins via the CHO-GSN™ platform
| Catalog # | Name | Timeline | Price |
|---|---|---|---|
| 1168 | 0.1 L transient production in HEK293 cells (Tuna293™ process) and Anti-CH1 affinity chromatography purification/Anti-IgM affinity chromatography purification | 2 weeks | $2,340 |
| 1176 | 0.1 L transient production in HEK293 cells (Tuna293™ process) and IMAC purification/Blue Sepharose/Immobilized Glutathione purification | 3 weeks | $2,490 |
| 1179 | 1.0 L transient production in HEK293 cells (Tuna293™ process) and IMAC purification/Blue Sepharose/Immobilized Glutathione purification | 3 weeks | $8,950 |
| 1355 | 0.1 L transient production in CHO cells (TunaCHO™ extended 14-day process) and Anti-CH1 affinity chromatography purification/Anti-IgM affinity chromatography purification | 3 weeks | $3,280 |
| 1363 | 0.1 L transient production in CHO cells (TunaCHO™ extended 14-day process) and IMAC purification/Blue Sepharose purification/Immobilized Glutathione purification | 4 weeks | $3,230 |
| 1366 | 1.0 L transient production in CHO cells (TunaCHO™ extended 14-day process) and IMAC purification/Blue Sepharose purification/Immobilized Glutathione purification | 4 weeks | $10,896 |
| 1749 | 0.1 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Anti-CH1 affinity chromatography purification/Anti-IgM affinity chromatography purification | 2 weeks | $2,340 |
| 1759 | 0.1 L transient production in CHO cells (TunaCHO™ standard 7-day process) and IMAC purification/Blue Sepharose purification/Immobilized Glutathione purification | 3 weeks | $2,490 |
| 1762 | 1.0 L transient production in CHO cells (TunaCHO™ standard 7-day process) and IMAC purification/Blue Sepharose purification/Immobilized Glutathione purification | 3 weeks | $8,950 |
| 1798 | Molecular construction of Fc fusion expression vector, 0.03L transient production in CHO cells (TunaCHO™ extended 14-day process) and Protein A purification (template provided) | 5-6 weeks | $2,600 |
| 1799 | Molecular construction of small peptide tag protein expression vector, 0.1 L transient production in CHO cells (TunaCHO™ extended 14-day process) and IMAC purification (template provided) | 6 weeks | $4,330 |
| 1800 | Molecular construction of Fc fusion expression vector, 0.03 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Protein A purification (template provided) | 4-5 weeks | $2,030 |
| 1801 | Molecular construction of small peptide tag protein expression vector, 0.1 L transient production in CHO cells (TunaCHO™ standard 7-day process) and IMAC purification (template provided) | 5 weeks | $3,590 |
| 27120 | Molecular construction of Fc fusion expression vector, 0.03 L transient production in HEK cells (Tuna293™ standard 7-day process) and Protein A purification (template provided) | 5 weeks | $2,030 |
| 27308 | Molecular construction of small peptide tag protein expression vector, 0.1 L transient production in HEK cells (Tuna293™ standard 7-day process), IMAC purification (template provided) | 5 weeks | $3,440 |