Catalog #27120 - Molecular construction of Fc fusion expression vector, 0.03 L transient production in HEK cells (Tuna293™ standard 7-day process) and Protein A purification (template provided)

Title:
Molecular construction of Fc fusion expression vector, 0.03 L transient production in HEK cells (Tuna293™ standard 7-day process) and Protein A purification (template provided)

Description:

Molecular construction of Fc fusion expression vector (template provided)

- DNA scale-up sufficient for 0.03 L production (1 DNA), includes DNA sequence confirmation

- 0.03 L transient production in HEK293 cells (Tuna293™ process)

- Protein A purification, CE-SDS analysis QC is included


Scope:

Molecular construction only services:

Protein genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

Scale-up productions are available at an additional cost


Packages for molecular construction and small scale production:

1. Protein genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

2. Pilot small-scale transient transfection into HEK293 cells (Tuna293™ Process) or CHO cells (TunaCHO™ process) is performed, followed by the listed purification method to assess expression levels and protein yield.


Deliverable:

Molecular construction only services:

-plasmid DNA (if in a commercially available vector)

OR

-plasmid DNA used in scale-up productions at LakePharma


Packages for molecular construction and small scale production:

1. Purified protein

2. Study Report

3. Certificate of Analysis


Please note that plasmid DNA will not be provided if LakePharma's vector system is utilized.


Material Required:

1. Sequence of VH and VL

2. DNA template (if DNA synthesis is not desired)


Timeline:
5 weeks

Price:
$2,132