Molecular Construction and Small Scale Protein Production

LakePharma provides plasmid design and construction services for the production of recombinant proteins. LakePharma’s in-house proprietary vector system ensures compatibility with the Tuna expression system for transient protein production in HEK293 cells.

For proteins, multiple small peptide backbones for molecular construction are available as well as larger tags such as GST.

Please see Tuna293 transient production pages for protein scale-up production services. 

Cat.# Service Name Timeline Size Price Quantity Request
27120 Molecular construction of Fc fusion expression vector, 0.03 liter transient production in HEK293 cells (Tuna293™ Process) and protein A purification (gene synthesis is not included) 4-5 weeks 0.03 liter $1950
27308 Molecular construction of small peptide tag protein expression vector, 0.1 liter transient production in HEK293 cells (Tuna293™ Process) and His tag purification (gene synthesis is not include) 5 weeks 0.1 liter $3150
Custom Molecular construction of small peptide affinity tag expression vector, 0.1 liter transient production in HEK293 cells (Tuna293™ Process) and affinity purification (gene synthesis not included) 4-5 weeks 0.1 liter Inquire

Molecular construction only services:

Protein genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

Scale-up productions are available at an additional cost

 

Packages for molecular construction and small scale production:

1. Protein genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

2. Pilot small-scale (0.03 liter) transient transfection into HEK293 cells (Tuna293™ Process) is performed, followed by the listed purification method to assess expression levels and protein yield.

1. Sequence of VH and VL

2. DNA template (if DNA synthesis is not desired)

Molecular construction only services:

-plasmid DNA (if in a commercially available vector)

OR

-plasmid DNA used in scale-up productions at LakePharma

 

Packages for molecular construction and small scale production:

1. Purified protein

2. Study Report

3. Certificate of Analysis

 

Please note that plasmid DNA will not be provided if LakePharma's vector system is utilized.