Molecular Construction and Small Scale Protein Production

LakePharma provides plasmid design and construction services for the production of recombinant proteins. LakePharma’s in-house proprietary vector system ensures compatibility with Tuna293™ Process or TunaCHO™ Process for transient production in HEK293 or CHO cells.

For proteins, multiple small peptide backbones for molecular construction are available as well as larger tags such as GST.

For TunaCHO, choose standard 7-day production for quick production cycle or 14-day extended production for higher yield.  Click here to learn more about TunaCHO performance.

Please contact LakePharma at inquiries@lakepharma.com for custom quotes and details.

Cat.# Service Name Timeline Size Price Quantity Request
27120 Molecular construction of Fc fusion expression vector, 0.03 liter transient production in HEK293 cells (Tuna293™ Process) and protein A purification (gene synthesis not included) 4-5 weeks 0.03 liter $2030
27308 Molecular construction of His tag protein expression vector, 0.1 liter transient production in HEK293 cells (Tuna293™ Process) and His tag purification (gene synthesis not include) 4-5 weeks 0.1 liter $3440
1800 Molecular construction of Fc fusion expression vector, 0.03 liter transient production in CHO cells (TunaCHO™ Process, 7-day standard production), protein A purification (gene synthesis not included) 4-5 weeks 0.03 liter $2030
1801 Molecular construction of His tag expression vector, 0.1 liter transient production in CHO cells (TunaCHO™ Process, 7-day standard production) and His tag purification (gene synthesis not included) 4-5 weeks 0.1 liter $3590
1798 Molecular construction of Fc fusion expression vector, 0.03 liter transient production in CHO cells (TunaCHO™ Process, 14-day extended production), protein A purification (gene synthesis not included) 5-6 weeks 0.03 liter $2600
1799 Molecular construction of His tag expression vector, 0.1 liter transient production in CHO cells (TunaCHO™ Process, 14-day extended production) and His tag purification (gene synthesis not included) 5-6 weeks 0.1 liter $4330

Molecular construction only services:

Protein genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

Scale-up productions are available at an additional cost

 

Packages for molecular construction and small scale production:

1. Protein genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

2. Pilot small-scale transient transfection into HEK293 cells (Tuna293™ Process) or CHO cells (TunaCHO™ process) is performed, followed by the listed purification method to assess expression levels and protein yield.

1. Sequence of VH and VL

2. DNA template (if DNA synthesis is not desired)

Molecular construction only services:

-plasmid DNA (if in a commercially available vector)

OR

-plasmid DNA used in scale-up productions at LakePharma

 

Packages for molecular construction and small scale production:

1. Purified protein

2. Study Report

3. Certificate of Analysis

 

Please note that plasmid DNA will not be provided if LakePharma's vector system is utilized.