Pichia pastoris offers many of the advantages of other eukaryotic systems, such as disulfide bond formation, proteolytic processing, protein folding, and glycosylation. It grows in relatively simple medium, lacks endotoxin, and genetic manipulations are straightforward. Pichia pastoris also has an efficient secretory pathway, so proteins can be produced in conditioned medium, simplifying downstream processing.
|1030||Yeast Scale-up Expression||Dependent on scale||Request|
|25210||Vector construction, plasmid production, and strain creation||15-20 days||Request|
|25220||50 ml small scale expression analysis with affinity pulldown||8-10 days||Request|
|25230||1 liter expression, and one-step affinity purification||2-3 weeks||Request|
|25240||1 liter expression, and tandem affinity purification||2-3 weeks||Request|
|25250||1 liter expression, tandem affinity purification, and an SEC polishing step||2-3 weeks||Request|