Viral Vector Services

The LakePharma Vector Center provides a comprehensive suite of viral vector services, including vector engineering, preclinical process development and scale-up production, and analytical development. Production systems offered include both baculovirus (AAV) and mammalian (AAV & Lentivirus). 

Available Viral Vector Systems

Baculovirus    
- Licensed partner for Oxford Expression Systems flashBAC™, Thermo Fisher Bac-to-Bac®, and BD BaculoGold™
- BIIC storage
- Suspension culture
- Rapid high titer production (2 weeks)
  - Third generation
- Four-plasmid system
- HEK293 production
  - Serotype agnostic
- Baculovirus and mammalian helper-free systems
- Adherent and suspension culture

 

Comprehensive Viral Vector Services

     
- Vector design
- Gene synthesis
- Genetic cloning
- Plasmid production
  - Baculovirus
- Mammalian
- Adherent and suspension culture
- Wave bioreactors (300 L)
  - High-pressure homogenization
- AKTA chromatography
- Affinity, IEX, SEC, etc.
- Diafiltration
- Ultracentrifugation
  - Capsid Titer (ELISA)
- Genome Titer (ddPCR)
- Infectious Titer (TCID)
- Purity (PAGE)
- Full:Empty Capsid (EM)
- Bioburden
- Mycoplasma (PCR)
- Host cell DNA and protein

 

For more information and custom quote, please email inquiries@lakepharma.com.