Viral Vectors & Cell Engineering

The LakePharma Vector Technology group offers a comprehensive suite of viral vector and cell engineering services, which cover vector engineering, process development, scale-up production, and analytical development. Viral vector platforms include Baculovirus, AAV, and Lentivirus. The cell engineering team performs stable cell line development and clone isolation for the production of recombinant proteins utilizing viral transduction and transfection methodologies.  The team has experience with a large variety of cell types, including CAR-T, and a host of other cell lines derived from various tissues.  

New! Presentation: LakePharma Viral Vector Center for Advanced Therapies 

Available Viral Vector Systems


 


 


- Bacmid system
- BIIC storage
- Suspension culture
- Rapid high titer production (2 weeks)		 - Third generation
- Four-plasmid system
- HEK293 production		 - Insect (Sf9; baculovirus)
- HEK293 helper-free system

 Comprehensive Viral Vector Services


 


 


 


 


- Vector design
- Gene synthesis
- Genetic cloning
- Plasmid production		 - Utilize viral vectors
- Variety of cell lines
- Primary cells (e.g., CAR-T)
 - Insect (Baculovirus) 
 - Mammalian 
- Adherent and suspension platforms
- Scale-up production		 - Depth filtration
- Chromatography
- Affinity, IEX, SEC etc.,
- Ultracentrifugation
- Diafiltration
 - Capsid titer (ELISA)
- Genome titer (ddPCR)
- Infectious titer
- Purity (PAGE)
- Full empty capsid (electron microscopy)
- Aggregation (DLS)
- Bioburden/sterility
- Mycoplasma (PCR)
- Host cell DNA/Protein

Case study: Purity and Identity Characterization of AAV Capsid Particles by LC-MS Methods in collaboration with Agilent