Genetic Assay Technology
Evaluation of DNA, RNA, miRNA, siRNA and other nucleic acids species is becoming more and more critical in Discovery, R&D and GMP production workflows; providing new evidence to link targets to disease and evaluation of efficacy, safety and commercial potential of therapeutics.
LakePharma provides support for your R&D and GxP nucleic acid analyses as part of our Genetic Assay Technology Suite of Services. Incorporating the latest technologies and instrumentation, we custom-design workflows to suit your data needs.
Highlights: (click on headers below for more details!)
Droplet Digital PCR (ddPCR) Workflows
- BioRad QX200 AutoDG System with Automated Plate Preparation
- Custom reagent design, testing and optimization as well as Commercially Available probe/primer assays
- Process Residual Assays, AAV and Lentiviral genome titers in both process samples for AAV/Lenti production as well as tissue/biofluid distribution assessment for potency assays.
- Sample preparation from Tissues, Cells, Biofluids and FFPE with QC.
- Multiple Sequence Detectors, Quanstudio 5s, Quanstudio 6 and Quanstudio 7 + Orbitor Plate Handler provide robust throughput focusing predominantly on 384-well format and Thermo TaqMan Fast chemistry.
- Custom reagent design, testing and optimization as well as Commercially Available probe/primer assays
- Small R&D workflows to Expansive Pre-Clinical Biomarker Studies with 1000s of samples and multiple GOI
- Sample preparation from Tissues, Cells, Biofluids and FFPE with QC.
- nCounter FLEX Analysis System with Dx Enablement and second automated prep station
- Direct, multiplexed digital barcode technology for quantifying DNA, RNA and protein simultaneously.
- Up to 800 analyte capabilities with very small sample input (100ng DNA/RNA)
- Pre-defined panels or custom configured reagents
- Sample preparation and analysis or fully customized upstream cell-based treatment workflows
- Sample preparation from Tissues, Cells, Biofluids and FFPE with QC
Next Generation Sequencing and RNAseq Sample Preparation and Quality Control Workflows
- Agilent TapeStation 4200 instrument for RIN and DV200 analysis
- Sample preparation from Tissues, Cells, Biofluids and FFPE with QC
- Fully customized upstream cell-based treatment workflows
- Multiple available NGS Sequencing Partners for Data Delivery to Client Informatics teams