The Baculovirus Expression Vector System (BEVS) is an established method within the scientific and industrial communities for generating large quantities of recombinant protein. BEVS is a eukaryotic system that utilizes the Autographa californica nuclear polyhedrosis virus (AcNPV) for high-level expression of recombinant proteins in Lepodoptera-derived insect cell lines, such as Sf9, Sf21, and High Five cells. Recombinant proteins produced using the system typically have correct folding, disulfide bond formation, and oligomerization, resulting in production of proteins that are functionally similar to their native forms. The system can accommodate large DNA inserts, perform intron/exon splicing, and yield relatively high levels of recombinant protein (0.1 to 50% of totalprotein). Short affinity tags, such as polyhistidine, Flag, and Twin-Strep, are often fused to recombinant proteins to aid in detection and purification of proteins. After affinity capture, tags can be removed using recombinant proteases such as TEV, HRV3C, thrombin, or SUMOStar.
LakePharma offers traditional and bacmid-based platforms for protein expression. Available platforms include Oxford Expression System flashBAC™, Bac-2-the-Future, and Thermo Fisher Bac-to-Bac®.