Catalog #65050

Title:
De Novo Antibody Sequencing I

Description:
de novo Antibody Sequencing I (Mass Spec results and analysis only)

Scope:

1. The sample is analyzed by LC-MS and CE-SDS to confirm purity and monoclonality of antibody sample.

2. The sample is digested by a panel of 5 enzymes specifically chosen to increase the probability of covering the entire sequence of an antibody sample. The resulting peptide mixture is submitted to LC-MS/MS analysis.

3. The mass spectra is analyzed using Valens, a proprietary data analysis platform for de novo sequencing.

4. Sequences identified are sent to the client. In the event full sequences are not identified, the full project price is still invoiced. Additional troubleshooting can be done for an additional fee.


Deliverable:
Package I: Interim report with LC-MS and CE-SDS results. Final study report with sequences (if obtained). Package II: Interim report with LC-MS and CE-SDS results. 1 mg of purified recombinant antibody. Final study report with sequences upon receipt of milestone payment.

Material Required:
0.5 mg of monoclonal antibody at ≥1 mg/mL and ≥95% purity. Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to LakePharma for analysis.

Timeline:
1 month

Price:
$15,000