CHO Transient Production (TunaCHO™ Process)

CHO cells have been the workhorse for protein and antibody manufacturing. The major advantages of CHO include robust growth, hardiness in suspension culture, stellar protein production and secretion, and a long track record. The biggest drawback of CHO is that the cells are hard to transfect. As such, CHO cells have been primarily used for CHO stable production, not transient.

TunaCHO technology platform solves the technical problem of low transient transfection efficiency. We are able to achieve a production yield greater than 1 gram per liter in shake flasks. We further have shown consistent PTM profiles between TunaCHO and CHO-GSN. Based on yields, LakePharma favors CHO over HEK293 as a transient protein production platform.

Below is a graph of typical yields between our Tuna293 transient production platform, our TunaCHO expression system, and other CHO transient expression system to show the difference in yields.



Recombinant antibody expression with TunaCHO process is significantly higher than HEK293 cells.

Download flyer: Large-scale Ab Production: Hybridoma or Recombinant?

To download our TunaCHO presentation click here.

A variety of purification methods and production scales are available. Sample pricing is shown below.


Catalog # Name Timeline Price
1335 0.1 L transient production in CHO cells (TunaCHO™ extended 14-day process) and Protein A purification 3 weeks $2,595
1336 0.25 L transient production in CHO cells (TunaCHO™ extended 14-day process) and Protein A purification 3 weeks $4,235
1338 1.0 L transient production in CHO cells (TunaCHO™ extended 14-day process) and Protein A purification 3 weeks $9,020
1738 0.03 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Protein A purification 2 weeks $1,134
1739 0.1 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Protein A purification 2 weeks $1,785
1740 0.25 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Protein A purification 2 weeks $3,080
1742 1.0 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Protein A purification 2 weeks $7,040
1788 0.03 L transient production in CHO cells (TunaCHO™ extended 14-day process) and Protein A purification 3 weeks $1,733