Catalog #1528 - High-throughput production service 96-well (TunaCHO™ 14-day process)

Title:
High-throughput production service 96-well (TunaCHO™ 14-day process)

Description:

High-throughput molecular construction (synthesize variable regions), DNA scale-up, 0.01 L transient production in CHO cells (TunaCHO™ 14-day process), and Protein A purification 

  • DNA sequence confirmation is included
  • Intact mass QC by mass spectrometry is included for standard human and mouse IgGs only

Scope:
  1. Molecular construction of IgG expression constructs in LakePharma’s proprietary vector (synthesize variable regions)
  2. DNA scale-ups (midiprep scale), including sequence confirmation
  3. 0.01 liter transient productions in CHO cells (HTP TunaCHO™ process)
  4. HTP Protein A purification
  5. Intact mass spec QC (reduced and deglycosylated conditions), complimentary for standard human and mouse IgGs only

Deliverable:
  • All purified protein in standard buffer and unadjusted concentration obtained from 0.01 liter productions, frozen in 96-well deep well plate
  • Study Report
  • Summary of yields and aliquots
  • Mass Spec report

Additional service options:  

Individual aliquots: $1200/plate


Material Required:

1. VH and VL sequences for 96 antibodies.

2. DNA template (if DNA synthesis is not desired).

3. Specified constant region for all 96 antibodies.


Note: One constant region for heavy chain and light chain must be specified for all 96 antibodies.


Timeline:
6-7 Weeks

Price:
$115,200