Antibody/Fc On-bead Conjugation

Immunoaffinity purification utilizes the highly specific binding properties of antibodies (or Fc-fusion proteins) to easily isolate ligands (or receptors) from crude mixtures with very high purities. Lakepharma offers the ability to attach antibodies or Fc-fusion proteins to Protein A/G resins and immobilize them using chemical crosslinkers to generate custom and stable immunoaffinity resins.

Please contact LakePharma at inquiries@lakepharma.com for custom quotes and details.

Cat.# Service Name Timeline Size Price Quantity Request
28200 Conjugation to ProA/G Resin 1 week < 1 mL resin $1400
Custom Conjugation to ProA/G Resin 1 week 1 - 10 mL resin $2240
Custom Conjugation to ProA/G Resin 1 week 11 - 50 mL resin $3584
Note: List prices do not include cost of resin.
  1. Conjugation of client provided proteins onto ProteinA/G resin targeting a client specified protein amount (mg) per mL of resin
  2. Removal on non-immobilized proteins of resin
  3. Additional leeching studies available for additional costs
  4. Buffer exchange into PBS; all other formulations may require an additional fee per sample
  5. Indirect quantitation of the average number of target proteins per mL of resin
  6. Low endotoxin processing available for additional cost
  1. Purified proteins for conjugation
  2. Resin
  3. Additionally, LakePharma can provide ProA/G resin for bundled services. Please inquire

 

  1. All custom conjugated resins
  2. Buffer formulation: PBS; all other formulations may require an additional fee per sample
  3. Protein concentration: Client specified
  4. Endotoxin levels: < 1 EU/mg if low endotoxin service is requested
  5. COA includes: Total volume  of custom resin, average amount of proteins per mL of resin, final buffer composition, endotoxin levels
  6. Standard aliquoting (up to 5 vials of equal volume, with the remainder in glass vials); Custom aliquoting available for additional costs
  7. Protein shipping temperature: 4°C