APC/ PE Conjugation

APC/ PE Conjugation

Allophycocyanin (APC) and Phycoerthyrin (PE) are two types of phycobiliproteins that are strongly fluorescent. Due to their high quantum efficiencies, large Stoke shifts, high water solubility, and stability under a wide range of pH and salt concentrations, APC and PE are commonly used fluorescent labels for a wide range of biological applications. LakePharma offers services to custom conjugate APC and PE to proteins at a specified Fluorophore-to-Protein ratio.

Please contact LakePharma at inquiries@lakepharma.com for custom quotes and details.

Cat.# Service Name Timeline Size Price Quantity Request
Custom APC/PE Conjugation 2 weeks < 5 mg proteins or antibodies $2600
Custom APC/PE Conjugation 2 weeks 5 - 20 mg proteins or antibodies $5000
Custom APC/PE Conjugation 2 weeks 21 - 50 mg proteins or antibodies $7800
Note: List prices do not include cost of APC/PE.
  1. APC/PE conjugation of client provided proteins targeting a client specified Fluorophore-to-Protein ratio
  2. Excess APC/PE removal services are available at additional costs
  3. Buffer exchange into PBS; all other formulations may require an additional fee per sample
  4. Quantitation of the average number of APC/PE molecules per protein
  5. Low endotoxin processing available for additional cost

Client Provided Materials:

  1. Purified proteins for labeling
  2. APC/PE proteins
  3. Additionally, LakePharma can provide activated APC/PE for labeling services with significant cost savings. Please inquire.
  1. All APC/PE conjugated proteins
  2. Buffer formulation: PBS; all other formulations may require an additional fee per sample
  3. Protein concentration: Client specified
  4. Endotoxin levels: < 1 EU/mg if low endotoxin service is requested
  5. COA includes: Final protein concentration, average number of APC/PE’s per protein ratio, final buffer composition, endotoxin levels
  6. Standard aliquoting (up to 5 vials of equal volume, with the remainder in glass vials); Custom aliquoting available for additional costs
  7. Protein shipping temperature: -80°C