Custom On-bead Conjugation

Affinity resins are utilized in pulldown assays, co-purifications, immunopurifications, and custom diagnostic platforms. Lakepharma offers on-bead conjugation services that will generate custom affinity resin at a variety of scales using a variety of chemistries that will expand your capabilities for highly custom affinity purification.

Please contact LakePharma at inquiries@lakepharma.com for custom quotes and details.

Cat.# Service Name Timeline Size Price Quantity Request
Custom Custom On-bead Conjugation TBD TBD TBD
  1. Conjugation of client provided proteins onto Sepharose resin targeting a client specified protein amount (mg) per mL of resin
  2. Removal of non-immobilized proteins from resin
  3. Additional leeching studies available for additional costs
  4. Buffer exchange into PBS; all other formulations may require an additional fee per sample
  5. Indirect quantitation of the average number of target proteins per mL of resin
  1. Purified proteins for conjugation
  2. Resin
  3. Additionally, LakePharma can provide agarose resin for conjugation services for additional costs. Please inquire
  4. Crosslinker reagents
  1. All custom conjugated resins
  2. Buffer formulation: PBS; all other formulations may require an additional fee per sample
  3. Protein concentration: Client specified
  4. Endotoxin levels: < 1 EU/mg if low endotoxin service is requested
  5. COA includes: Total volume  of custom resin, average amount of proteins per mL of resin, final buffer composition, endotoxin levels
  6. Standard aliquoting (up to 5 vials of equal volume, with the remainder in glass vials); Custom aliquoting available for additional costs
  7. Protein shipping temperature: 4°C