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Catalog #28310 - Fluorophore Labeling of 21-50 mg protein or antibodies
Title:
Fluorophore Labeling of 21-50 mg protein or antibodies
Description:
Fluorophore Labeling of 21-50 mg protein or antibodies
- [Amount of protein] of [Protein name] will be used for labeling
- [Name of Fluorophore] is the selected fluorophore
- Target Fluorophore-to-Protein ratio (F/P) of [Desired F/P ratio]
- Standard fluorophore labeling service
- QC F/P ratio using spectrophotometry
- Endotoxin requirement: [State the endotoxin requirement]
*Cost of fluorophore not included
Scope:
- Protein labeling with commercially available dyes derivatized with Succinimidyl-Esters or Maleimides targeting custom Fluorophore to Protein ratios (F/P)
- Excess dye removal using dialysis
- Buffer exchange into PBS; all other formulations may require an additional fee per sample
- F:P determination using spectrophotometry
- Low endotoxin processing is available for additional costs
Deliverable:
1.All labeled proteins
2. Buffer formulation: PBS; all other formulations may require an additional fee per sample
3. Protein concentration: Client specified
4. Endotoxin levels: < 1 EU/mg if low endotoxin service is requested
5. COA includes: Final protein concentration, average number of biotins per protein ratio, final buffer composition, endotoxin levels if ed
6. Standard aliquoting (up to 5 vials of equal volume, with the remainder in glass vials); Custom aliquoting available for additional costs
7. Protein shipping temperature: -80°C
Material Required:
- Purified proteins for labeling
- Fluorophores for labeling (A $60 procurement fee will be applied if LakePharma places the order for the reagents)