Catalog #28310 - Fluorophore Labeling of 21-50 mg protein or antibodies

Title:

Fluorophore Labeling of 21-50 mg protein or antibodies

Description:

Fluorophore Labeling of  21-50 mg protein or antibodies

- [Amount of protein] of [Protein name] will be used for labeling

- [Name of Fluorophore] is the selected fluorophore

- Target Fluorophore-to-Protein ratio (F/P) of  [Desired F/P ratio]

- Standard fluorophore labeling service

- QC F/P ratio using spectrophotometry

- Endotoxin requirement: [State the endotoxin requirement]

*Cost of fluorophore not included

Scope:

1. Protein labeling with commercially available dyes derivatized with Succinimidyl-Esters or Maleimides targeting custom Fluorophore to Protein ratios (F/P)
2. Excess dye removal using dialysis
3. Buffer exchange into PBS; all other formulations may require an additional fee per sample
4. F:P determination using spectrophotometry
5. Low endotoxin processing is available for additional costs

Deliverable:

1.All labeled proteins

2. Buffer formulation: PBS; all other formulations may require an additional fee per sample

3. Protein concentration: Client specified

4. Endotoxin levels: < 1 EU/mg if low endotoxin service is requested

5. COA includes: Final protein concentration, average number of biotins per protein ratio, final buffer composition, endotoxin levels if ed

6. Standard aliquoting (up to 5 vials of equal volume, with the remainder in glass vials); Custom aliquoting available for additional costs

7. Protein shipping temperature: -80°C

Material Required:

- Purified proteins for labeling 

- Fluorophores for labeling (A $60 procurement fee will be applied if LakePharma places the order for the reagents)

Timeline:

7 days

Price:

Request