In Vitro Glycoengineering (IVGE)

Glycosylation is a common type of post-translational modification that can significantly influence protein stability, biological activity, and pharmacokinetics of the final products. Therefore, one way to manipulate glycosylation is through IVGE. 

LakePharma is teaming up with Roche CustomBiotech to offer custom IVGE service designed to improve therapeutic protein production. With a two-step reaction of galactosylation followed by sialyation, glycoforms can be enriched with known kinetics and predictable outcomes.

Pre- and post- in vitro glycoengineering data showing enrichment of certain glycoforms

 

LakePharma's In Vitro Glycoengineering Services

LakePharma's in vitro glycoengineering service workflow

 

 

Key Features

 

Rapid IVGE process with high protein recovery
•  IVGE reactions can be completed in 2-3 days, with projects and data completed within 1-2 weeks
•  Minimal protein loss

     
 

Robust and consistent glycan conversion through IVGE
•  Lot-to-lot consistency for glycan conversion and protein recovery
•  Improved glycosylation and increased stability of therapeutic proteins observed after IVGE

     
 

Extensive expertise in glycoanalysis and other bioanalytical capabilities
•  In addition to glycan profiling and other glycan evaluations, additional bioanalytical analysis can also be bundled 

     
 

Dedicated analytical tools and equipment for evaluation of IVGE success and data verification
•  SE-UPLC via Waters® ACQUITY UPLC®
•  cIEF via ProteinSimple® iCE3™
•  Post-translational modification analysis via mass spec (Xevo® G2-XS, Waters)

     

 

        

 

Cat.# Service Name Timeline Size Price Quantity Request
2001 IVGE Pilot Run Inquire Inquire Request
2002 IVGE Optimization Run Inquire Inquire Request
  1. Original sample will be aliquoted for glycan profiling and characterization assays.
  2. The sample will undergo two steps of the reaction, galactosylation, and sialylation.
  3. Protein-to-enzyme ratios are determined within the "IVGE Process Optimization" step.
  4. Samples will be polished using an appropriate purification method.
  5. Samples after reaction will undergo glycan profiling and characterization assays for comparison.

IVGE Pilot Scale Run:

- 5 mg of purified protein at ≥ 2 mg/mL

- Sequence of target protein

- Theoretical pI value

- Purification method/tag on protein of interest

 

IVGE Process Optimization:

- 20 mg of purified protein at ≥ 2 mg/mL

- Sequence of target protein

- Theoretical pI value

- Purification method/tag on protein of interest

 

IVGE Scale Up:

- Client requested amount of purified protein at ≥ 2 mg/mL

- Sequence of target protein

- Theoretical pI value

- Purification method/tag on protein of interest

- Glycan Profiles

- Aggregation analysis report

- Purity analysis report

- Charge Variant Analysis report