In Vitro Glycoengineering (IVGE)
Glycosylation can significantly influence protein stability, biological activity, and pharmacokinetics of the final products. One way to manipulate glycosylation is through IVGE.
LakePharma is teaming up with Roche CustomBiotech to offer custom IVGE service designed to improve therapeutic protein production. With a two-step reaction of galactosylation followed by sialyation, glycoforms can be enriched with known kinetics and predictable outcomes.
LakePharma's In Vitro Glycoengineering Services
Key Features
| Rapid IVGE process with high protein recovery • IVGE reactions can be completed in 2-3 days,
with projects and data completed within 1-2 weeks •
Minimal protein loss |
| Robust and consistent glycan conversion through IVGE • Lot-to-lot consistency for glycan conversion
and protein recovery •
Improved glycosylation and increased stability of therapeutic proteins
observed after IVGE |
| Extensive expertise in glycoanalysis and
other bioanalytical capabilities • In addition to glycan profiling and other glycan evaluations, additional bioanalytical analysis can also be bundled |
| Dedicated analytical tools and
equipment for evaluation of IVGE success and data verification • SE-UPLC via Waters® ACQUITY UPLC® • cIEF via ProteinSimple® iCE3™
• Post-translational modification analysis via mass spec (Xevo® G2-XS,
Waters) |
| Catalog # | Name | Timeline | Price |
|---|---|---|---|
| 2001 | In vitro galactosylation and sialylation pilot analysis: | 1.5 weeks | Request |
| 2002 | In vitro galactosylation and sialylation optimization analysis: | 3 weeks | Request |
| 2003 | In Vitro Glycoengineering Scale Up | Request |
