In Vitro Glycoengineering (IVGE)
Glycosylation is a common type of post-translational modification that can significantly influence protein stability, biological activity, and pharmacokinetics of the final products. Therefore, one way to manipulate glycosylation is through IVGE.
LakePharma is teaming up with Roche CustomBiotech to offer custom IVGE service designed to improve therapeutic protein production. With a two-step reaction of galactosylation followed by sialyation, glycoforms can be enriched with known kinetics and predictable outcomes.
LakePharma's In Vitro Glycoengineering Services
Key Features
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Rapid IVGE process with high protein recovery |
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Robust and consistent glycan conversion through IVGE |
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| Cat.# | Service Name | Timeline | Size | Price | Quantity | Request |
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- Original sample will be aliquoted for glycan profiling and characterization assays.
- The sample will undergo two steps of the reaction, galactosylation, and sialylation.
- Protein-to-enzyme ratios are determined within the "IVGE Process Optimization" step.
- Samples will be polished using an appropriate purification method.
- Samples after reaction will undergo glycan profiling and characterization assays for comparison.
IVGE Pilot Scale Run:
- 5 mg of purified protein at ≥ 2 mg/mL
- Sequence of target protein
- Theoretical pI value
- Purification method/tag on protein of interest
IVGE Process Optimization:
- 20 mg of purified protein at ≥ 2 mg/mL
- Sequence of target protein
- Theoretical pI value
- Purification method/tag on protein of interest
IVGE Scale Up:
- Client requested amount of purified protein at ≥ 2 mg/mL
- Sequence of target protein
- Theoretical pI value
- Purification method/tag on protein of interest
- Glycan Profiles
- Aggregation analysis report
- Purity analysis report
- Charge Variant Analysis report