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Catalog #2002 - In vitro galactosylation and sialylation optimization analysis:
Title:
In vitro galactosylation and sialylation optimization analysis:
Description:
In vitro galactosylation and sialylation optimization analysis:
- There are two steps of a reaction to optimize.
- Different enzyme to protein ratio will be evaluated for each step.
- GalT1, 1:50 (1 mg)
- GalT1, 1:200 (1 mg)
- GalT1, 1:50, ST6, 1:10 (4.5 mg)
- GalT1, 1:50, ST6, 1:50 (4.5 mg)
- GalT1, 1:200, ST6, 1:10 (4.5 mg)
- GalT1, 1:200, ST6, 1:50 (4.5 mg)
- There will be 6 samples for proA purification after the reaction and analytical testing.
- Mass spec, CE-SDS, cIEF, and SE-UPLC on each sample
Scope:
- Original sample will be aliquoted for glycan profiling and characterization assays.
- The sample will undergo two steps of the reaction, galactosylation, and sialylation.
- Protein-to-enzyme ratios are determined within the "IVGE Process Optimization" step.
- Samples will be polished using an appropriate purification method.
- Samples after reaction will undergo glycan profiling and characterization assays for comparison.
Deliverable:
- Glycan Profiles- Aggregation analysis report- Purity analysis report- Charge Variant Analysis report
Material Required:
- 20 mg of purified protein at ≥ 2 mg/mL
- Sequence of target protein
- Theoretical pI value
- Purification method/tag on protein of interest
