- Molecular Biology
- Viral Vectors & Cell Engineering
- Protein Engineering & Production
- Antibody Discovery & Engineering
- Antibody Production
-
Analytics & Formulation
- Overview
- Purity Analysis by CE-SDS
- Aggregation Analysis
- Charge Variant Analysis
- De Novo Antibody Sequencing
- Intact Mass Analysis
- Sequence Confirmation by Mass Spectrometry
- N-terminal Sequencing
- Post-Translational Modification
- N-glycan Analysis
- Thermostability Analysis
- Stability Analysis
- Formulation Development
- Developability Analysis
- FAQs
- R&D and cGxP Bioassay Development
- Protein Chemistry
- Development & Manufacturing in LakePharma Hayward, CA
- Development & Manufacturing in LakePharma Hopkinton, MA
- Consulting Services
Cell Engineering
The cell engineering team performs stable cell line development and clone isolation for the production of recombinant proteins utilizing viral transduction and transfection methodologies. The team has experience with a large variety of cell types, including CAR-T, and a host of other cell lines derived from various tissues. Transgene expression is analyzed by various methods, such as flow cytometry, western blot, and ELISA.
Key features of Cell Engineering Services
- Variety of cell types
- HEK293, CHO, A549, hematopoietic (T cells, including CAR-T)
- Hundreds of engineered cell lines created
- Target types
- Enzymes, antibodies, membrane/structural proteins, reporter proteins, etc.
- Antibiotic titration (kill curve) analysis
- Gene delivery
- Viral vector-mediated transduction
- Cationic lipid transfection
- Single-cell cloning
- Research cell banks
Click here to download the viral vector services presentation
Case study: Purity and Identity Characterization of AAV Capsid Particles by LC-MS Methods in collaboration with Agilent
